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KMID : 0613820100200020281
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Journal of Life Science
2010 Volume.20 No. 2 p.281 ~ p.291
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Antioxidant Effect of Hederagenin 3-O-b-D-Glucopyranosyl(1→3)-a-L-Rhamnopyranosyl(1→2)-a-L-Arabinopyranoside (HDL) Isolated from Root Bark of Ulmus davidiana
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Bong Jin-Gu
Park Yoon-Yub
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Abstract
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We investigated the antioxidant effects of hederagenin 3-O-b-D-glucopyranosyl(1→3)-a-L-rhamnopyranosyl(1→2)-a-L-arabinopyranoside (HDL) isolated from root bark of Ulmus davidiana on the activity of enzymes related to reactive oxygen species (ROS) in human osteosarcoma U2OS cells. Cobalt chloride (CoCl₂), a transition metal, was used as an inducer of oxidative stress, generating hydrogen peroxide (H₂O₂) via increasing xanthine oxidase (XO) activity. The increased levels of H₂O₂, XO, ferritin, and ferritin iron by CoCl₂ were diminished effectively by co-treatment with HDL in U2OS cells. Furthermore, decreased levels of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) by CoCl2 were highly increased by co-treatment with HDL in U2OS cells; however, the levels of glutathione peroxidase (GPx) did not change. The increased contents of TBARS related to lipid peroxidation were significantly reduced by HDL in U2OS cells. The concentration of GSH changed in a pattern that went against regulated TBARS by CoCl₂ and HDL. We examined the expression of p53, p21CIP1/WAF1, and p27KIP1 proteins related to oxidative stress and cell cycle regulation. As a result, the expression of p27KIP1 modulated by CoCl₂ was not changed by HDL. However, the expression of p53 and p21CIP1/WAF increased by CoCl₂ was reduced by HDL in U2OS cells. Together with alteration of p53 and p21CIP1/WAF1 proteins, the accumulated cells at G1 phase by CoCl₂ was decreased by HDL in U2OS cells. Our data suggests that HDL inhibits CoCl₂-generated ROS in U2OS cells, providing potentially new antioxidant compounds that are isolated from natural products.
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KEYWORD
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Hederagenin 3-O-b-D-glucopyranosyl(1→3)-a-L-rhamnopyranosyl(1→2)-a-L-arabinopyranoside(HDL), Ulmus davidiana, reactive oxygen species (ROS), antioxidant, cell cycle
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